| Summary: | The objective of this work was to review the status of citrus in Paraguay and to evaluate the biotechnological strategies applied to obtain plants free of pathogens. There are two main research centers in Paraguay: The Paraguayan Institute of Agrarian Technology (IPTA) and the Faculty of Agrarian Sciences of the National University of Asuncion (UNA), through which the main diseases are reported. These include psorosis, cancrosis, the citrus sadness virus (CTV) and the Huanglongbing virus (HLB). Through the Citrus Improvement Program, disease-free plants have been obtained by applying biotechnological techniques, mainly the in vitro shoot apex micrografting, which allows the development of a more efficient and productive citrus crop, in short periods of time compared with the conventional techniques.
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