STREPTOCOCCUS PNEUMONIAE PENICILLIN SUSCEPTIBILITY BY DIFUSSION TECHNIQUE. COMPARISON OF TWO METHODS
Pneumococcal Infections caused by drug-resistant strains are an increasing problem requiring the adoption of control strategies and campaigns to promote judicious use of antibiotics. To screen for pneumococcal penicillin resistance using the Kirby-Bauer disc technique with oxacillin impregnated disc...
| Main Authors: | , |
|---|---|
| Format: | Online |
| Language: | Spanish |
| Published: |
Facultad de Ciencias Exactas, Químicas y Naturales
2003
|
| Subjects: | |
| Online Access: | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/402 |
| _version_ | 1813809303140696064 |
|---|---|
| author | von Specht, M. H. Grenon, S. L. |
| author_facet | von Specht, M. H. Grenon, S. L. |
| author_sort | von Specht, M. H. |
| collection | Revista de Ciencia y Tecnologia RECyT |
| description | Pneumococcal Infections caused by drug-resistant strains are an increasing problem requiring the adoption of control strategies and campaigns to promote judicious use of antibiotics. To screen for pneumococcal penicillin resistance using the Kirby-Bauer disc technique with oxacillin impregnated discs, the National Committee for Clinical Laboratory Standards (NCCLS) recommends Mueller Hinton Agar supplemented with 5% defibrinated sheep blood (MHSO). Since that supplement is not accessible for many laboratories, routine antibiotic sensitivity tests are not performed in many hospitals, and thus empirical therapy could be inappropriate. The aim of this research is to compare two in vitro disk diffusion techniques to determine Streptococcus pneumoniae penicillin susceptibility. Seventy four strains were used in this study. The technique and the interpretative breakpoint were in accordance with NCCLS criteria for both methods run in parallel, except for the use of 5% serologically typed human blood as supplement to MH agar in the alternative method (MHSH). |
| format | Online |
| id | ojs-article-402 |
| institution | Facultad de Ciencias Exactas Quimicas y Naturales |
| language | Spanish |
| publishDate | 2003 |
| publisher | Facultad de Ciencias Exactas, Químicas y Naturales |
| record_format | ojs |
| spelling | ojs-article-4022020-03-06T00:42:00Z STREPTOCOCCUS PNEUMONIAE PENICILLIN SUSCEPTIBILITY BY DIFUSSION TECHNIQUE. COMPARISON OF TWO METHODS DETERMINACIÓN DE LA SENSIBILIDAD DE STREPTOCOCCUS PNEUMONIAE A PENICILINA POR TÉCNICA DE DIFUSIÓN. COMPARACIÓN DE DOS MÉTODOS von Specht, M. H. Grenon, S. L. Streptococcus pneumoniae oxacillin difussion Streptococcus pneumoniae oxacilina difusión Pneumococcal Infections caused by drug-resistant strains are an increasing problem requiring the adoption of control strategies and campaigns to promote judicious use of antibiotics. To screen for pneumococcal penicillin resistance using the Kirby-Bauer disc technique with oxacillin impregnated discs, the National Committee for Clinical Laboratory Standards (NCCLS) recommends Mueller Hinton Agar supplemented with 5% defibrinated sheep blood (MHSO). Since that supplement is not accessible for many laboratories, routine antibiotic sensitivity tests are not performed in many hospitals, and thus empirical therapy could be inappropriate. The aim of this research is to compare two in vitro disk diffusion techniques to determine Streptococcus pneumoniae penicillin susceptibility. Seventy four strains were used in this study. The technique and the interpretative breakpoint were in accordance with NCCLS criteria for both methods run in parallel, except for the use of 5% serologically typed human blood as supplement to MH agar in the alternative method (MHSH). Las infecciones por neumococos resistentes constituyen un problema creciente que exige medidas de control, y campañas para promover el uso racional de los antibióticos. Para establecer la sensibilidad a penicilina (PEN) por difusión, según Kirby Bauer, el National Commitee for Clinical Laboratory Standards (NCCLS) recomienda la utilización de Agar Mueller Hinton suplementado con sangre de carnero al 5% (MHSO). Como este suplemento no resulta fácilmente accesible, muchos laboratorios no efectúan esta determinación y se recurre a terapias empíricas a veces inadecuadas. El objetivo del trabajo fue realizar una comparación entre dos medios de cultivo aplicados al método de difusión para la determinación de la sensibilidad a penicilina de S. Pneumoniae, utilizando discos de oxacilina. Se efectuó el estudio en paralelo a 74 cepas. Se siguieron las normas del NCCLS para el desarrollo de la técnica e interpretación de los halos obtenidos, siendo la única variable el suplemento con sangre humana (serológicamente controlada) al 5% como método propuesto (MHSH). Facultad de Ciencias Exactas, Químicas y Naturales 2003-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/402 Argentine Journal of Science and Technology; Vol. 5 No. 1 (2003); 12-16 Revista de Ciencia y Tecnología; Vol. 5 Núm. 1 (2003); 12-16 1851-7587 0329-8922 spa https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/402/324 Derechos de autor 2003 Revista de Ciencia y Tecnología |
| spellingShingle | Streptococcus pneumoniae oxacillin difussion Streptococcus pneumoniae oxacilina difusión von Specht, M. H. Grenon, S. L. STREPTOCOCCUS PNEUMONIAE PENICILLIN SUSCEPTIBILITY BY DIFUSSION TECHNIQUE. COMPARISON OF TWO METHODS |
| title | STREPTOCOCCUS PNEUMONIAE PENICILLIN SUSCEPTIBILITY BY DIFUSSION TECHNIQUE. COMPARISON OF TWO METHODS |
| title_alt | DETERMINACIÓN DE LA SENSIBILIDAD DE STREPTOCOCCUS PNEUMONIAE A PENICILINA POR TÉCNICA DE DIFUSIÓN. COMPARACIÓN DE DOS MÉTODOS |
| title_full | STREPTOCOCCUS PNEUMONIAE PENICILLIN SUSCEPTIBILITY BY DIFUSSION TECHNIQUE. COMPARISON OF TWO METHODS |
| title_fullStr | STREPTOCOCCUS PNEUMONIAE PENICILLIN SUSCEPTIBILITY BY DIFUSSION TECHNIQUE. COMPARISON OF TWO METHODS |
| title_full_unstemmed | STREPTOCOCCUS PNEUMONIAE PENICILLIN SUSCEPTIBILITY BY DIFUSSION TECHNIQUE. COMPARISON OF TWO METHODS |
| title_short | STREPTOCOCCUS PNEUMONIAE PENICILLIN SUSCEPTIBILITY BY DIFUSSION TECHNIQUE. COMPARISON OF TWO METHODS |
| title_sort | streptococcus pneumoniae penicillin susceptibility by difussion technique comparison of two methods |
| topic | Streptococcus pneumoniae oxacillin difussion Streptococcus pneumoniae oxacilina difusión |
| topic_facet | Streptococcus pneumoniae oxacillin difussion Streptococcus pneumoniae oxacilina difusión |
| url | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/402 |
| work_keys_str_mv | AT vonspechtmh streptococcuspneumoniaepenicillinsusceptibilitybydifussiontechniquecomparisonoftwomethods AT grenonsl streptococcuspneumoniaepenicillinsusceptibilitybydifussiontechniquecomparisonoftwomethods AT vonspechtmh determinaciondelasensibilidaddestreptococcuspneumoniaeapenicilinaportecnicadedifusioncomparaciondedosmetodos AT grenonsl determinaciondelasensibilidaddestreptococcuspneumoniaeapenicilinaportecnicadedifusioncomparaciondedosmetodos |
Epidemiology of Pneumococcal Surface Protein a (PspA) IN Streptococcus pneumoniae Causing Invasive Infections in a Paediatric Hospital in Argentina