Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance
Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina.
| Main Authors: | , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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BioMed Central
2020
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| Online Access: | https://hdl.handle.net/20.500.12219/5078 |
| _version_ | 1814555074609807360 |
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| author | Roldán Gallardo, Franco Fabián Dematteo, Karen E. Rinas, Miguel Ángel Argüelles, Carina Francisca |
| author_facet | Roldán Gallardo, Franco Fabián Dematteo, Karen E. Rinas, Miguel Ángel Argüelles, Carina Francisca |
| author_sort | Roldán Gallardo, Franco Fabián |
| collection | Repositorio RIDUNaM |
| description | Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina. |
| format | info:eu-repo/semantics/article |
| id | ir-20.500.12219-5078 |
| institution | Universidad Nacional de Misiones |
| language | English |
| publishDate | 2020 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | ir-20.500.12219-50782024-06-04T12:55:50Z Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance Roldán Gallardo, Franco Fabián Dematteo, Karen E. Rinas, Miguel Ángel Argüelles, Carina Francisca Cuniculus paca Heterologous amplifcation Microsatellite primers Saliva Scat Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina. Fil: Roldán Gallardo, Franco Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical; Argentina. Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Fil: Dematteo, Karen E. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina. Fil: Dematteo, Karen E. Wildcare Institute at the Saint Louis Zoo; Estados Unidos. Fil: Dematteo, Karen E. Washington University in St. Louis. Department of Biology & Environmental Studies; Estados Unidos. Fil: Rinas, Miguel Ángel. Ministerio de Ecología y Recursos. Naturales Renovables (Misiones); Argentina. Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina. Fil: Argüelles, Carina Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical; Argentina. Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Objective: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplifcation of 20 heterologous microsatellite primer pairs developed for guinea pigs (Cavia porcellus) and capybara (Hydrochoerus hydrochaeris). Results: Those primers that showed the best amplifcation profle in blood samples were subsequently applied to scats and saliva samples, to evaluate their efciency. Of the 13 microsatellite pairs that amplifed in blood, one-third (32%) were successfully amplifed in saliva and scat samples. This initial work demonstrates successful cross-amplif- cation in paca providing a solid and promising foundation for future genetic studies with this species. The ability to quantify genetic diversity using noninvasive samples from free-ranging paca is essential to developing applied management strategies for this large neotropical rodent that is not only a prey favored by wide-ranging carnivores [e.g., jaguar (Panthera onca), puma (Puma concolor)], but is also a species targeted by illegal hunting and wildlife trade. 2020-07-07 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://hdl.handle.net/20.500.12219/5078 eng info:eu-repo/semantics/altIdentifier/hdl/http://hdl.handle.net/11336/140665 info:eu-repo/semantics/altIdentifier/urn/https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-020-05312-x info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s13104-020-05312-x info:eu-repo/semantics/openAccess application/pdf application/pdf 1012 KB BioMed Central |
| spellingShingle | Cuniculus paca Heterologous amplifcation Microsatellite primers Saliva Scat Roldán Gallardo, Franco Fabián Dematteo, Karen E. Rinas, Miguel Ángel Argüelles, Carina Francisca Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance |
| title | Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance |
| title_full | Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance |
| title_fullStr | Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance |
| title_full_unstemmed | Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance |
| title_short | Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance |
| title_sort | heterologous microsatellite primers are informative for paca cuniculus paca a large rodent with economic and ecological importance |
| topic | Cuniculus paca Heterologous amplifcation Microsatellite primers Saliva Scat |
| url | https://hdl.handle.net/20.500.12219/5078 |
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Genetic characterization of maize landraces from Misiones, Argentina