Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse
Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
| Main Authors: | , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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Taylor & Francis
2019
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| Online Access: | https://hdl.handle.net/20.500.12219/5428 |
| _version_ | 1815466082496937984 |
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| author | Díaz, Gabriela Verónica Zapata, Pedro Darío Villalba, Laura Lidia Fonseca, María Isabel |
| author_facet | Díaz, Gabriela Verónica Zapata, Pedro Darío Villalba, Laura Lidia Fonseca, María Isabel |
| author_sort | Díaz, Gabriela Verónica |
| collection | Repositorio RIDUNaM |
| description | Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. |
| format | info:eu-repo/semantics/article |
| id | ir-20.500.12219-5428 |
| institution | Universidad Nacional de Misiones |
| language | English |
| publishDate | 2019 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | ir-20.500.12219-54282024-06-19T15:20:55Z Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse Díaz, Gabriela Verónica Zapata, Pedro Darío Villalba, Laura Lidia Fonseca, María Isabel Aspergillus Endoxylanases B-xylosidases Proteases Sugarcane bagasse Multivariate analysis Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Zapata, Pedro Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Villalba, Laura Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Fonseca, María Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Fil: Fonseca, María Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina. Xylanases hydrolyse efficiently the xylan component of hemicelluloses into single xylose monomers. Hence, these enzymes are suitable to be applied in the bioconversion of lignocellulosic wastes such as sugarcane bagasse to improve the bioethanol process. Misiones rainforest in the northeast of Argentina has a huge biodiversity which becomes the most likely site to screen new fungal isolates for powerful xylanolytic enzymes. The aim of this work was to find efficient xylanolytic Aspergillus with low levels of proteolytic enzymes through the isolation and exploration of native fungi from Misiones. Fungi were isolated from different locations of this subtropical region and 25 Aspergillus isolates were screened based on their ability to produce xylanolytic and proteolytic enzymes. From the principal component and conglomerate analysis, six groups were discriminated. The most promising fungi presented the highest endoxylanase and b-xylosidase activities and the lowest proteolytic activities. These isolates were A. niger LBM 055 and A. niger LBM 134 which reached 29 and 27 UmL1 of endoxylanases, respectively; 0.6 UmL1 of b-xylosidases, no casein and very little gelatin proteases. This study proves that the search of new fungi from nature is an important strategy to find novel isolates with the ability to secrete high-performance xylanases and exploit these enzymes in the bioconversion of lignocellulosic wastes. 2019-06-01 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://hdl.handle.net/20.500.12219/5428 eng info:eu-repo/semantics/altIdentifier/hdl/http://hdl.handle.net/11336/107324 info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1080/25765299.2019.1622922 info:eu-repo/semantics/altIdentifier/urn/https://www.tandfonline.com/doi/epdf/10.1080/25765299.2019.1622922?needAccess=true info:eu-repo/semantics/openAccess application/pdf application/pdf 1.8 MB Taylor & Francis |
| spellingShingle | Aspergillus Endoxylanases B-xylosidases Proteases Sugarcane bagasse Multivariate analysis Díaz, Gabriela Verónica Zapata, Pedro Darío Villalba, Laura Lidia Fonseca, María Isabel Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse |
| title | Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse |
| title_full | Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse |
| title_fullStr | Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse |
| title_full_unstemmed | Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse |
| title_short | Evaluation of new xylanolytic-producing isolates of Aspergillus from Misiones subtropical rainforest using sugarcane bagasse |
| title_sort | evaluation of new xylanolytic producing isolates of aspergillus from misiones subtropical rainforest using sugarcane bagasse |
| topic | Aspergillus Endoxylanases B-xylosidases Proteases Sugarcane bagasse Multivariate analysis |
| url | https://hdl.handle.net/20.500.12219/5428 |
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In vitro preliminary assessment of aflatoxin ability of Aspergillus flavus from peanuts