Pretreatment soda-ethanol of pine and its influence on enzymatic hydrolysis
The production of second-generation bioethanol using pine sawdust involves pretreatment, enzymatic hydrolysis (EH) and fermentation. One of the most influencing factor in the cellulose hydrolysis is the chemical composition of the substrate, which is directly linked with the kind and conditions of t...
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Format: | Online |
Language: | Spanish |
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Facultad de Ciencias Exactas, Químicas y Naturales
2017
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Online Access: | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/202 |
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author | Kruyeniski, Julia Felissia, Fernando E. Area, María C. |
author_facet | Kruyeniski, Julia Felissia, Fernando E. Area, María C. |
author_sort | Kruyeniski, Julia |
collection | Revista de Ciencia y Tecnologia RECyT |
description | The production of second-generation bioethanol using pine sawdust involves pretreatment, enzymatic hydrolysis (EH) and fermentation. One of the most influencing factor in the cellulose hydrolysis is the chemical composition of the substrate, which is directly linked with the kind and conditions of the applied pretreatment to biomass. The aim of this work was to study the enzymatic digestibility of pine sawdust submitted to alkaline - ethanol pretreatments. In the experiments, we varied alkaline charges and time at maximum temperature. Laboratory Analytical Procedures (LAPs,NREL) were used for the chemical characterization of the material, the enzymatic activity and the hydrolysis. The pretreated materials presented different chemical compositions. Only the effect of alkaline charge on yield was significant. Ethanol and soda exhibited a positive synergy effect on yield. Results suggest the existence of a negative correlation between EH yield and lignin content. |
format | Online |
id | ojs-article-202 |
institution | Facultad de Ciencias Exactas Quimicas y Naturales |
language | Spanish |
publishDate | 2017 |
publisher | Facultad de Ciencias Exactas, Químicas y Naturales |
record_format | ojs |
spelling | ojs-article-2022020-03-06T10:56:19Z Pretreatment soda-ethanol of pine and its influence on enzymatic hydrolysis Pretratamiento soda-etanol de pino y su influencia en la hidrólisis enzimática Kruyeniski, Julia Felissia, Fernando E. Area, María C. Deslignificación Hidrólisis Enzimática Aserrín de pino Pretratamiento organosolv Bioetanol Delignification Enzymatic Hydrolysis Pine Sawdust Organosolv Pretreatment Bioethanol The production of second-generation bioethanol using pine sawdust involves pretreatment, enzymatic hydrolysis (EH) and fermentation. One of the most influencing factor in the cellulose hydrolysis is the chemical composition of the substrate, which is directly linked with the kind and conditions of the applied pretreatment to biomass. The aim of this work was to study the enzymatic digestibility of pine sawdust submitted to alkaline - ethanol pretreatments. In the experiments, we varied alkaline charges and time at maximum temperature. Laboratory Analytical Procedures (LAPs,NREL) were used for the chemical characterization of the material, the enzymatic activity and the hydrolysis. The pretreated materials presented different chemical compositions. Only the effect of alkaline charge on yield was significant. Ethanol and soda exhibited a positive synergy effect on yield. Results suggest the existence of a negative correlation between EH yield and lignin content. La obtención de bioetanol de segunda generación a partir de aserrín de pino incluye: pretratamiento, hidrólisis enzimática (HE) y fermentación. Uno de los factores más influyentes en la hidrólisis es la composición química del sustrato, directamente relacionada con las condiciones y tipo de pretratamiento al cual es sometido el material. El objetivo fue estudiar la digestibilidad enzimática del aserrín de pino sometido a un pretratamiento etanol-soda. En el experimento se varió la carga alcalina y el tiempo a temperatura máxima. Para la caracterización del material, la actividad enzimática y la hidrólisis se usaron los Procedimientos Analíticos de Laboratorio (LAPs, NREL). Los materiales pretratados presentaron diferente composición química. Solo el efecto de la carga alcalina sobre el rendimiento de la HE fue significativo. Etanol y soda exhibieron una sinergia positiva con respecto al rendimiento. Los resultados sugieren una correlación negativa entre el rendimiento de la HE y el contenido de lignina. Facultad de Ciencias Exactas, Químicas y Naturales 2017-12-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/202 Argentine Journal of Science and Technology; Vol. 28 No. 1 (2017) Revista de Ciencia y Tecnología; Vol. 28 Núm. 1 (2017) 1851-7587 0329-8922 spa https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/202/291 |
spellingShingle | Deslignificación Hidrólisis Enzimática Aserrín de pino Pretratamiento organosolv Bioetanol Delignification Enzymatic Hydrolysis Pine Sawdust Organosolv Pretreatment Bioethanol Kruyeniski, Julia Felissia, Fernando E. Area, María C. Pretreatment soda-ethanol of pine and its influence on enzymatic hydrolysis |
title | Pretreatment soda-ethanol of pine and its influence on enzymatic hydrolysis |
title_alt | Pretratamiento soda-etanol de pino y su influencia en la hidrólisis enzimática |
title_full | Pretreatment soda-ethanol of pine and its influence on enzymatic hydrolysis |
title_fullStr | Pretreatment soda-ethanol of pine and its influence on enzymatic hydrolysis |
title_full_unstemmed | Pretreatment soda-ethanol of pine and its influence on enzymatic hydrolysis |
title_short | Pretreatment soda-ethanol of pine and its influence on enzymatic hydrolysis |
title_sort | pretreatment soda ethanol of pine and its influence on enzymatic hydrolysis |
topic | Deslignificación Hidrólisis Enzimática Aserrín de pino Pretratamiento organosolv Bioetanol Delignification Enzymatic Hydrolysis Pine Sawdust Organosolv Pretreatment Bioethanol |
topic_facet | Deslignificación Hidrólisis Enzimática Aserrín de pino Pretratamiento organosolv Bioetanol Delignification Enzymatic Hydrolysis Pine Sawdust Organosolv Pretreatment Bioethanol |
url | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/202 |
work_keys_str_mv | AT kruyeniskijulia pretreatmentsodaethanolofpineanditsinfluenceonenzymatichydrolysis AT felissiafernandoe pretreatmentsodaethanolofpineanditsinfluenceonenzymatichydrolysis AT areamariac pretreatmentsodaethanolofpineanditsinfluenceonenzymatichydrolysis AT kruyeniskijulia pretratamientosodaetanoldepinoysuinfluenciaenlahidrolisisenzimatica AT felissiafernandoe pretratamientosodaetanoldepinoysuinfluenciaenlahidrolisisenzimatica AT areamariac pretratamientosodaetanoldepinoysuinfluenciaenlahidrolisisenzimatica |