Effects of pH, temperature and incubation time on fungal growth and laccase activity in Trametes villosa BAFC 2755
White rot fungi, such as Trametes villosa BAFC 2755, are the only organisms capable of efficiently degrading lignin due to their oxidative enzymatic system. One of the most important enzymes in this process is laccase. This is a phenol oxidase, which has applications in several biotechnological proc...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | Spanish |
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Facultad de Ciencias Exactas, Químicas y Naturales
2019
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| Subjects: | |
| Online Access: | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/276 |
| _version_ | 1810374999302733824 |
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| author | Barchuk, Mónica Lucrecia Fonseca, María I. Giorgio, Ernesto M. Zapata, Pedro D. |
| author_facet | Barchuk, Mónica Lucrecia Fonseca, María I. Giorgio, Ernesto M. Zapata, Pedro D. |
| author_sort | Barchuk, Mónica Lucrecia |
| collection | Revista de Ciencia y Tecnologia RECyT |
| description | White rot fungi, such as Trametes villosa BAFC 2755, are the only organisms capable of efficiently degrading lignin due to their oxidative enzymatic system. One of the most important enzymes in this process is laccase. This is a phenol oxidase, which has applications in several biotechnological processes including pulp delignification, detoxification of recalcitrant chemicals, degradation of polycyclic aromatic compounds, bioremediation of contaminated soils and waters. The objective of this work was to evaluate the effect of pH, temperature and incubation time on the growth and oxidative potential of T. villosa BAFC 2755, for that were carried out a quantitative of tests in culture medium ME and activity in lacasa were determinated, to complete the analysis a gene fragment of this enzyme were characterize. The results obtained showed that the conditions that improved biomass production were not those that supported the greatest activity laccase. Maximun activity levels (200 U/L), were recorded after 14 days, at pH 5.5 and 25° C. Lacase’s isoenzyme profile was affected by pH, temperature and culture time, with three isoenzymes of 64 kDa, 123 kDa and 132 kDa. In addition, a nucleotide sequence of 779 bp was obtained from a gene coding for lacase, whose protein sequence showed 90% identity with sequences belonging to the lacase gene of other species of Trametes sp. |
| format | Online |
| id | ojs-article-276 |
| institution | Facultad de Ciencias Exactas Quimicas y Naturales |
| language | Spanish |
| publishDate | 2019 |
| publisher | Facultad de Ciencias Exactas, Químicas y Naturales |
| record_format | ojs |
| spelling | ojs-article-2762020-03-09T13:46:37Z Effects of pH, temperature and incubation time on fungal growth and laccase activity in Trametes villosa BAFC 2755 Efectos de pH, temperatura y tiempo de incubación sobre el crecimiento fúngico y la actividad lacasa en Trametes villosa BAFC 2755 Barchuk, Mónica Lucrecia Fonseca, María I. Giorgio, Ernesto M. Zapata, Pedro D. enzymatic activity fungal biomass white rot fungus isoenzymes Trametes Lacasa Isoenzimas Biomasa fúngica White rot fungi, such as Trametes villosa BAFC 2755, are the only organisms capable of efficiently degrading lignin due to their oxidative enzymatic system. One of the most important enzymes in this process is laccase. This is a phenol oxidase, which has applications in several biotechnological processes including pulp delignification, detoxification of recalcitrant chemicals, degradation of polycyclic aromatic compounds, bioremediation of contaminated soils and waters. The objective of this work was to evaluate the effect of pH, temperature and incubation time on the growth and oxidative potential of T. villosa BAFC 2755, for that were carried out a quantitative of tests in culture medium ME and activity in lacasa were determinated, to complete the analysis a gene fragment of this enzyme were characterize. The results obtained showed that the conditions that improved biomass production were not those that supported the greatest activity laccase. Maximun activity levels (200 U/L), were recorded after 14 days, at pH 5.5 and 25° C. Lacase’s isoenzyme profile was affected by pH, temperature and culture time, with three isoenzymes of 64 kDa, 123 kDa and 132 kDa. In addition, a nucleotide sequence of 779 bp was obtained from a gene coding for lacase, whose protein sequence showed 90% identity with sequences belonging to the lacase gene of other species of Trametes sp. Los hongos de pudrición blanca, como Trametes villosa BAFC 2755, son los únicos organismos capaces de degradar eficientemente la lignina debido a que poseen un sistema enzimático oxidativo. Una de las enzimas más importantes en este proceso es la lacasa. Esta es una fenoloxidasa, que posee aplicaciones en varios procesos biotecnológicos incluyendo la deslignificación de la pulpa de papel, detoxificación de químicos recalcitrantes, degradación de compuestos policíclicos aromáticos, biorremediación de suelos y aguas contaminadas. El presente trabajo tuvo como objetivo evaluar el efecto del pH, la temperatura y el tiempo de incubación sobre el crecimiento y el potencial oxidativo de T. villosa BAFC 2755. Para ello se llevaron a cabo ensayos en medio de cultivo ME y se determinó la actividad lacasa. Para complementar el análisis de caracterizó una región del gen que codifica para una lacasa. Los resultados obtenidos mostraron que las condiciones que mejoraron la producción de biomasa no fueron las que soportaron la mayor actividad lacasa. Los máximos niveles de actividad se registraron a 25° C al día 14 para pH 5,5 siendo de 200 U/L. El perfil de isoenzimas de lacasa fue afectado por el pH, la temperatura y el tiempo de cultivo, encontrándose tres isoenzimas de 64 kDa, 123 kDa y 132 kDa. Además, se obtuvo una secuencia nucleotídica de 779 pb de un gen que codifica para lacasa, cuya secuencia proteica mostró identidad de 90% con secuencias pertenecientes al gen lacasa de otras especies de Trametes sp. Facultad de Ciencias Exactas, Químicas y Naturales 2019-11-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/276 10.36995/j.recyt.2019.32.015 Argentine Journal of Science and Technology; Vol. 32 No. 1 (2019); 91-98 Revista de Ciencia y Tecnología; Vol. 32 Núm. 1 (2019); 91-98 1851-7587 0329-8922 10.36995/j.recyt.2019.32 spa https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/276/480 https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/276/516 Derechos de autor 2019 Revista de Ciencia y Tecnología |
| spellingShingle | enzymatic activity fungal biomass white rot fungus isoenzymes Trametes Lacasa Isoenzimas Biomasa fúngica Barchuk, Mónica Lucrecia Fonseca, María I. Giorgio, Ernesto M. Zapata, Pedro D. Effects of pH, temperature and incubation time on fungal growth and laccase activity in Trametes villosa BAFC 2755 |
| title | Effects of pH, temperature and incubation time on fungal growth and laccase activity in Trametes villosa BAFC 2755 |
| title_alt | Efectos de pH, temperatura y tiempo de incubación sobre el crecimiento fúngico y la actividad lacasa en Trametes villosa BAFC 2755 |
| title_full | Effects of pH, temperature and incubation time on fungal growth and laccase activity in Trametes villosa BAFC 2755 |
| title_fullStr | Effects of pH, temperature and incubation time on fungal growth and laccase activity in Trametes villosa BAFC 2755 |
| title_full_unstemmed | Effects of pH, temperature and incubation time on fungal growth and laccase activity in Trametes villosa BAFC 2755 |
| title_short | Effects of pH, temperature and incubation time on fungal growth and laccase activity in Trametes villosa BAFC 2755 |
| title_sort | effects of ph temperature and incubation time on fungal growth and laccase activity in trametes villosa bafc 2755 |
| topic | enzymatic activity fungal biomass white rot fungus isoenzymes Trametes Lacasa Isoenzimas Biomasa fúngica |
| topic_facet | enzymatic activity fungal biomass white rot fungus isoenzymes Trametes Lacasa Isoenzimas Biomasa fúngica |
| url | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/276 |
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Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones