Optimisation of the enzymatic hydrolysis of rice husk
The objective of this work was to find the optimal conditions of enzymatic hydrolysis of the rice husk pretreated with sodium hydroxide in an autoclave at 121° C. Screening design showed that all the selected variables (FPU / g substrate, pH, agitation, time, temperature and concentration of surfact...
| Main Authors: | , , , , |
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| Format: | Online |
| Language: | Spanish |
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Facultad de Ciencias Exactas, Químicas y Naturales
2019
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| Subjects: | |
| Online Access: | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/300 |
| _version_ | 1824325798695272448 |
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| author | Arismendy Pabon, Ana Maria Villa Restrepo, Andrés Felipe Alcaraz Zapata, Wilman Chamorro, Ester R. Area, Maria C. |
| author_facet | Arismendy Pabon, Ana Maria Villa Restrepo, Andrés Felipe Alcaraz Zapata, Wilman Chamorro, Ester R. Area, Maria C. |
| author_sort | Arismendy Pabon, Ana Maria |
| collection | Revista de Ciencia y Tecnologia RECyT |
| description | The objective of this work was to find the optimal conditions of enzymatic hydrolysis of the rice husk pretreated with sodium hydroxide in an autoclave at 121° C. Screening design showed that all the selected variables (FPU / g substrate, pH, agitation, time, temperature and concentration of surfactant) were statistically significant. Then an optimization design was applied for the variables: pH, FPU and time, discarding the ones with the lowest level of significance. Finally, the best conditions found in the previous designs were (pH 5.0, 13 h, and 30 FPU / g of substrate) subsequently these conditions were applied in experiences to evaluate the influence of the increase in hydrolysable cellulose, using 6%; 8% and 10% of cellulose available giving saccharification yields of 74%, 42% and 16%. |
| format | Online |
| id | ojs-article-300 |
| institution | Facultad de Ciencias Exactas Quimicas y Naturales |
| language | Spanish |
| publishDate | 2019 |
| publisher | Facultad de Ciencias Exactas, Químicas y Naturales |
| record_format | ojs |
| spelling | ojs-article-3002020-03-09T13:58:32Z Optimisation of the enzymatic hydrolysis of rice husk Optimización de la hidrólisis enzimática de la cascarilla de arroz Arismendy Pabon, Ana Maria Villa Restrepo, Andrés Felipe Alcaraz Zapata, Wilman Chamorro, Ester R. Area, Maria C. Rice husk Enzymatic hydrolysis Lignocellulosic Biomass Optimal Conditions Bioethanol Cascarilla de arroz Hidrólisis enzimática Biomasa Lignocelulósica Condiciones óptimas Bioetanol The objective of this work was to find the optimal conditions of enzymatic hydrolysis of the rice husk pretreated with sodium hydroxide in an autoclave at 121° C. Screening design showed that all the selected variables (FPU / g substrate, pH, agitation, time, temperature and concentration of surfactant) were statistically significant. Then an optimization design was applied for the variables: pH, FPU and time, discarding the ones with the lowest level of significance. Finally, the best conditions found in the previous designs were (pH 5.0, 13 h, and 30 FPU / g of substrate) subsequently these conditions were applied in experiences to evaluate the influence of the increase in hydrolysable cellulose, using 6%; 8% and 10% of cellulose available giving saccharification yields of 74%, 42% and 16%. El objetivo de este trabajo fue encontrar las condiciones óptimas de hidrólisis enzimática de la cascarilla de arroz pretratada con hidróxido de sodio en autoclave a 121° C. Se evaluaron las variables: FPU/ g sustrato, pH, agitación, tiempo, temperatura y concentración de surfactante mediante un diseño de cribado, resultando estadísticamente significativas todas las variables. Seguidamente se aplicó un diseño de optimización para las variables: pH, FPU y tiempo, descartando las de menor nivel de significación. Finalmente, las mejores condiciones encontradas en los diseños anteriores fueron (pH 5.0, 13 h, y 30 FPU / g de sustrato) posteriormente se aplicaron estas condiciones en experiencias para evaluar la influencia del aumento de celulosa hidrolizable, usando 6%; 8% y 10% de celulosa disponible con rendimientos de sacarificación de 74%, 42% y 16%. Facultad de Ciencias Exactas, Químicas y Naturales 2019-10-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/300 10.36995/j.recyt.2019.32.010 Argentine Journal of Science and Technology; Vol. 32 No. 1 (2019); 64-70 Revista de Ciencia y Tecnología; Vol. 32 Núm. 1 (2019); 64-70 1851-7587 0329-8922 10.36995/j.recyt.2019.32 spa https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/300/477 https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/300/511 Derechos de autor 2019 Revista de Ciencia y Tecnología |
| spellingShingle | Rice husk Enzymatic hydrolysis Lignocellulosic Biomass Optimal Conditions Bioethanol Cascarilla de arroz Hidrólisis enzimática Biomasa Lignocelulósica Condiciones óptimas Bioetanol Arismendy Pabon, Ana Maria Villa Restrepo, Andrés Felipe Alcaraz Zapata, Wilman Chamorro, Ester R. Area, Maria C. Optimisation of the enzymatic hydrolysis of rice husk |
| title | Optimisation of the enzymatic hydrolysis of rice husk |
| title_alt | Optimización de la hidrólisis enzimática de la cascarilla de arroz |
| title_full | Optimisation of the enzymatic hydrolysis of rice husk |
| title_fullStr | Optimisation of the enzymatic hydrolysis of rice husk |
| title_full_unstemmed | Optimisation of the enzymatic hydrolysis of rice husk |
| title_short | Optimisation of the enzymatic hydrolysis of rice husk |
| title_sort | optimisation of the enzymatic hydrolysis of rice husk |
| topic | Rice husk Enzymatic hydrolysis Lignocellulosic Biomass Optimal Conditions Bioethanol Cascarilla de arroz Hidrólisis enzimática Biomasa Lignocelulósica Condiciones óptimas Bioetanol |
| topic_facet | Rice husk Enzymatic hydrolysis Lignocellulosic Biomass Optimal Conditions Bioethanol Cascarilla de arroz Hidrólisis enzimática Biomasa Lignocelulósica Condiciones óptimas Bioetanol |
| url | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/300 |
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Pretreatment soda-ethanol of pine and its influence on enzymatic hydrolysis