Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones

Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones

The white rot fungi secrete oxidative enzymes, such as laccase, with a capacity to play a part in environmentally friendly processes such as bioremediation. The aim of this study was to develop a culture medium supplemented with aromatic compounds and metal ions in order to increase laccase activity...

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Bibliographic Details
Main Authors: Ortellado, Laura E., Fonseca, María I., Barchuk, Mónica L., Sawostjanik Afanasiuk, Silvina S., Villalba, Laura L., Zapata, Pedro D.
Format: Online
Language:Spanish
Published: Facultad de Ciencias Exactas, Químicas y Naturales 2016
Subjects:
Laccase activity
White-rot fungi
Trametes
Isozymes
Actividad lacasa
Hongos de pudrición blanca
Isoenzimas
Online Access:https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/333
Description
Summary:The white rot fungi secrete oxidative enzymes, such as laccase, with a capacity to play a part in environmentally friendly processes such as bioremediation. The aim of this study was to develop a culture medium supplemented with aromatic compounds and metal ions in order to increase laccase activity produced by Trametes sp. strain A. By means of a Plackett-Burman design, it was shown that Mn2 + was the only variable that had a significant positive effect on the enzyme activity. The highest laccase activity (272.46 U / L) was obtained at a concentration of 1.5 mM Mn2 + and maximum biomass (0.1272 g) at a concentration of 4 mM Mn2 +. It also was possible to establish a negative correlation between enzyme activity and growth of fungus. An isoenzyme of constitutive expression (60 kDa) and another inducible (100 kDa) were found in the presence of 2; 2.5; 3; 3.5 and 4 mM of Mn2+.

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