Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones

The white rot fungi secrete oxidative enzymes, such as laccase, with a capacity to play a part in environmentally friendly processes such as bioremediation. The aim of this study was to develop a culture medium supplemented with aromatic compounds and metal ions in order to increase laccase activity...

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Main Authors:	Ortellado, Laura E., Fonseca, María I., Barchuk, Mónica L., Sawostjanik Afanasiuk, Silvina S., Villalba, Laura L., Zapata, Pedro D.
Format:	Online
Language:	Spanish
Published:	Facultad de Ciencias Exactas, Químicas y Naturales 2016
Subjects:	Laccase activity White-rot fungi Trametes Isozymes Actividad lacasa Hongos de pudrición blanca Isoenzimas
Online Access:	https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/333

id	ojs-article-333
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spelling	ojs-article-3332020-03-06T10:55:50Z Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones Efecto de iones metálicos y compuestos aromáticos sobre la actividad lacasa en Trametes sp. nativo de Misiones Ortellado, Laura E. Fonseca, María I. Barchuk, Mónica L. Sawostjanik Afanasiuk, Silvina S. Villalba, Laura L. Zapata, Pedro D. Laccase activity White-rot fungi Trametes Isozymes Actividad lacasa Hongos de pudrición blanca Trametes Isoenzimas The white rot fungi secrete oxidative enzymes, such as laccase, with a capacity to play a part in environmentally friendly processes such as bioremediation. The aim of this study was to develop a culture medium supplemented with aromatic compounds and metal ions in order to increase laccase activity produced by Trametes sp. strain A. By means of a Plackett-Burman design, it was shown that Mn2 + was the only variable that had a significant positive effect on the enzyme activity. The highest laccase activity (272.46 U / L) was obtained at a concentration of 1.5 mM Mn2 + and maximum biomass (0.1272 g) at a concentration of 4 mM Mn2 +. It also was possible to establish a negative correlation between enzyme activity and growth of fungus. An isoenzyme of constitutive expression (60 kDa) and another inducible (100 kDa) were found in the presence of 2; 2.5; 3; 3.5 and 4 mM of Mn2+. Los hongos de pudrición blanca secretan enzimas oxidativas, como la lacasa, con capacidad de intervenir en procesos amigables con el medioambiente, tales como la biorremediación. El objetivo del presente trabajo fue formular un medio de cultivo suplementado con compuestos aromáticos e iones metálicos con el fin de aumentar los valores de actividad lacasa expresados por Trametes sp. cepa A. Utilizando un diseño de Plackett-Burman se logró establecer que el Mn2+ fue la única variable que tuvo un efecto significativo positivo sobre la actividad. La mayor actividad lacasa (272,46 U/L) se obtuvo a una concentración de 1,5 mM de Mn2+ y la máxima biomasa (0,1272 g) a una concentración de 4 mM de Mn2+. Asimismo fue posible establecer una correlación negativa entre la actividad enzimática y el crecimiento del hongo. Se encontró una isoenzima de 60 kDa de expresión constitutiva y otra de 100 kDa inducible en presencia de 2; 2,5; 3; 3,5 y 4 mM de Mn2+. Facultad de Ciencias Exactas, Químicas y Naturales 2016-12-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/333 Argentine Journal of Science and Technology; Vol. 26 No. 1 (2016); 4-10 Revista de Ciencia y Tecnología; Vol. 26 Núm. 1 (2016); 4-10 1851-7587 0329-8922 spa https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/333/235 Derechos de autor 2019 Revista de Ciencia y Tecnología
institution	Facultad de Ciencias Exactas Quimicas y Naturales
collection	Revista de Ciencia y Tecnologia RECyT
language	Spanish
topic	Laccase activity White-rot fungi Trametes Isozymes Actividad lacasa Hongos de pudrición blanca Trametes Isoenzimas
spellingShingle	Laccase activity White-rot fungi Trametes Isozymes Actividad lacasa Hongos de pudrición blanca Trametes Isoenzimas Ortellado, Laura E. Fonseca, María I. Barchuk, Mónica L. Sawostjanik Afanasiuk, Silvina S. Villalba, Laura L. Zapata, Pedro D. Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones
topic_facet	Laccase activity White-rot fungi Trametes Isozymes Actividad lacasa Hongos de pudrición blanca Trametes Isoenzimas
format	Online
author	Ortellado, Laura E. Fonseca, María I. Barchuk, Mónica L. Sawostjanik Afanasiuk, Silvina S. Villalba, Laura L. Zapata, Pedro D.
author_facet	Ortellado, Laura E. Fonseca, María I. Barchuk, Mónica L. Sawostjanik Afanasiuk, Silvina S. Villalba, Laura L. Zapata, Pedro D.
author_sort	Ortellado, Laura E.
title	Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones
title_short	Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones
title_full	Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones
title_fullStr	Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones
title_full_unstemmed	Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones
title_sort	effect of metal ions and aromatic compounds on laccase activity in trametes sp. native of misiones
title_alt	Efecto de iones metálicos y compuestos aromáticos sobre la actividad lacasa en Trametes sp. nativo de Misiones
description	The white rot fungi secrete oxidative enzymes, such as laccase, with a capacity to play a part in environmentally friendly processes such as bioremediation. The aim of this study was to develop a culture medium supplemented with aromatic compounds and metal ions in order to increase laccase activity produced by Trametes sp. strain A. By means of a Plackett-Burman design, it was shown that Mn2 + was the only variable that had a significant positive effect on the enzyme activity. The highest laccase activity (272.46 U / L) was obtained at a concentration of 1.5 mM Mn2 + and maximum biomass (0.1272 g) at a concentration of 4 mM Mn2 +. It also was possible to establish a negative correlation between enzyme activity and growth of fungus. An isoenzyme of constitutive expression (60 kDa) and another inducible (100 kDa) were found in the presence of 2; 2.5; 3; 3.5 and 4 mM of Mn2+.
publisher	Facultad de Ciencias Exactas, Químicas y Naturales
publishDate	2016
url	https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/333
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Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones

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