| Summary: | Caffeine is a substance derivate of purine basis that has many benefits, has been widely studied in areas related to health and human performance. In this sense, this work aims to simulate the oxidation of leaves of yerba mate, a process used to obtain the black tea from the Camellia sinensis leaves and monitor these to their caffeine content for HPLC. A factorial design of experiments was performed for five variables: temperature, humidity and light camera, moisture and age leaf. The results indicate that caffeine tend to decrease their content (%) with increasing leaf age. Making clear that 6,5 months is the intermediate age of the leaf whose values of caffeine are also intermediate.
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The labeling on the "yerba mate" (ilex paraguariensis) packages in the Mercosur" (south american common market)