Laboratory evaluation of a submerged system production of endo-1.4-B-glucanase secreted for two strains of white rot fungi
Cellulose is the main component of the cell wall with great biotechnological importance regarding the analysis of new cellulase producers microorganisms. The objective of this study was the design and testing of a submerged production system of endo-B-1.4-glucanase using pine chips as substrate; pH...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Online |
| Language: | Spanish |
| Published: |
Facultad de Ciencias Exactas, Químicas y Naturales
2013
|
| Subjects: | |
| Online Access: | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/428 |
| _version_ | 1810375033385648128 |
|---|---|
| author | Giorgio, Ernesto M. Morales, Andrea Congost, Gabriela Fonseca, María I. Abate, Carlos Zapata, Pedro D. Villalba, Laura L. |
| author_facet | Giorgio, Ernesto M. Morales, Andrea Congost, Gabriela Fonseca, María I. Abate, Carlos Zapata, Pedro D. Villalba, Laura L. |
| author_sort | Giorgio, Ernesto M. |
| collection | Revista de Ciencia y Tecnologia RECyT |
| description | Cellulose is the main component of the cell wall with great biotechnological importance regarding the analysis of new cellulase producers microorganisms. The objective of this study was the design and testing of a submerged production system of endo-B-1.4-glucanase using pine chips as substrate; pH and T optimum action of the enzyme extracts by two white rot fungi Irpex sp. and Phlebia sp. were checked at laboratory scale. The production system proposed in this study showed a logarithmic behaviour with respect to enzyme production with maximum activity of 1 U / Lh for Irpex sp.strain and 1.37 U / Lh for Phlebia sp. strain. Enzymatic activity remained constant over time through washing and adding fresh medium, simulating a fed-batch production system. Extracts of both strains showed better performance at 50°C, with higher activity at pH 3 for extracts of Irpex sp. strain and pH 4.5 for Phlebia sp. strain. This system may be optimal to start scaling up studies. |
| format | Online |
| id | ojs-article-428 |
| institution | Facultad de Ciencias Exactas Quimicas y Naturales |
| language | Spanish |
| publishDate | 2013 |
| publisher | Facultad de Ciencias Exactas, Químicas y Naturales |
| record_format | ojs |
| spelling | ojs-article-4282020-03-06T10:54:20Z Laboratory evaluation of a submerged system production of endo-1.4-B-glucanase secreted for two strains of white rot fungi Elaboración en laboratorio de un sistema sumergido de producción de Endo-1,4-B-Glucanasa secretada por dos cepas de hongos de pudrición blanca Giorgio, Ernesto M. Morales, Andrea Congost, Gabriela Fonseca, María I. Abate, Carlos Zapata, Pedro D. Villalba, Laura L. White Rot Fungi submerged fermentation system endo-1.4-B-glucanase Hongos de pudrición blanca sistema de sustrato sumergido endo-1,4-B-glucanasa Cellulose is the main component of the cell wall with great biotechnological importance regarding the analysis of new cellulase producers microorganisms. The objective of this study was the design and testing of a submerged production system of endo-B-1.4-glucanase using pine chips as substrate; pH and T optimum action of the enzyme extracts by two white rot fungi Irpex sp. and Phlebia sp. were checked at laboratory scale. The production system proposed in this study showed a logarithmic behaviour with respect to enzyme production with maximum activity of 1 U / Lh for Irpex sp.strain and 1.37 U / Lh for Phlebia sp. strain. Enzymatic activity remained constant over time through washing and adding fresh medium, simulating a fed-batch production system. Extracts of both strains showed better performance at 50°C, with higher activity at pH 3 for extracts of Irpex sp. strain and pH 4.5 for Phlebia sp. strain. This system may be optimal to start scaling up studies. La celulosa es el componente principal de la pared celular y es de gran importancia biotecnológica analizar nuevos microorganismos que produzcan celulasas. El objetivo de este trabajo fue diseñar y ensayar a nivel laboratorio un sistema de producción sumergido que utiliza chips de pino como sustrato para la producción de endo-1,4-B-glucanasa secretada por dos hongos de pudrición blanca, Irpex sp. y Phlebia sp. y verificar el pH y T óptimos de acción de los extractos enzimáticos. El sistema de producción propuesto en este trabajo mostró un comportamiento logarítmico respecto de la producción enzimática con un máximo de actividad de 1 U/L.h para Irpex sp. y 1,37 U/L.h para Phlebia sp. La actividad se mantuvo constante con el tiempo a través de lavados y agregado de medio fresco, simulando un sistema de producción fed-batch. Los extractos de ambas cepas mostraron una mejor performance a 50ºC, con una mayor actividad a pH 3 para los extractos de la cepa de Irpex sp. y a pH 4,5 para la cepa de Phlebia sp. Este sistema podría ser óptimo para iniciar los experimentos de escalado. Facultad de Ciencias Exactas, Químicas y Naturales 2013-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/428 Argentine Journal of Science and Technology; Vol. 19 No. 1 (2013); 18-23 Revista de Ciencia y Tecnología; Vol. 19 Núm. 1 (2013); 18-23 1851-7587 0329-8922 spa https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/428/350 Derechos de autor 2013 Revista de Ciencia y Tecnología |
| spellingShingle | White Rot Fungi submerged fermentation system endo-1.4-B-glucanase Hongos de pudrición blanca sistema de sustrato sumergido endo-1,4-B-glucanasa Giorgio, Ernesto M. Morales, Andrea Congost, Gabriela Fonseca, María I. Abate, Carlos Zapata, Pedro D. Villalba, Laura L. Laboratory evaluation of a submerged system production of endo-1.4-B-glucanase secreted for two strains of white rot fungi |
| title | Laboratory evaluation of a submerged system production of endo-1.4-B-glucanase secreted for two strains of white rot fungi |
| title_alt | Elaboración en laboratorio de un sistema sumergido de producción de Endo-1,4-B-Glucanasa secretada por dos cepas de hongos de pudrición blanca |
| title_full | Laboratory evaluation of a submerged system production of endo-1.4-B-glucanase secreted for two strains of white rot fungi |
| title_fullStr | Laboratory evaluation of a submerged system production of endo-1.4-B-glucanase secreted for two strains of white rot fungi |
| title_full_unstemmed | Laboratory evaluation of a submerged system production of endo-1.4-B-glucanase secreted for two strains of white rot fungi |
| title_short | Laboratory evaluation of a submerged system production of endo-1.4-B-glucanase secreted for two strains of white rot fungi |
| title_sort | laboratory evaluation of a submerged system production of endo 1 4 b glucanase secreted for two strains of white rot fungi |
| topic | White Rot Fungi submerged fermentation system endo-1.4-B-glucanase Hongos de pudrición blanca sistema de sustrato sumergido endo-1,4-B-glucanasa |
| topic_facet | White Rot Fungi submerged fermentation system endo-1.4-B-glucanase Hongos de pudrición blanca sistema de sustrato sumergido endo-1,4-B-glucanasa |
| url | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/428 |
| work_keys_str_mv | AT giorgioernestom laboratoryevaluationofasubmergedsystemproductionofendo14bglucanasesecretedfortwostrainsofwhiterotfungi AT moralesandrea laboratoryevaluationofasubmergedsystemproductionofendo14bglucanasesecretedfortwostrainsofwhiterotfungi AT congostgabriela laboratoryevaluationofasubmergedsystemproductionofendo14bglucanasesecretedfortwostrainsofwhiterotfungi AT fonsecamariai laboratoryevaluationofasubmergedsystemproductionofendo14bglucanasesecretedfortwostrainsofwhiterotfungi AT abatecarlos laboratoryevaluationofasubmergedsystemproductionofendo14bglucanasesecretedfortwostrainsofwhiterotfungi AT zapatapedrod laboratoryevaluationofasubmergedsystemproductionofendo14bglucanasesecretedfortwostrainsofwhiterotfungi AT villalbalaural laboratoryevaluationofasubmergedsystemproductionofendo14bglucanasesecretedfortwostrainsofwhiterotfungi AT giorgioernestom elaboracionenlaboratoriodeunsistemasumergidodeproducciondeendo14bglucanasasecretadapordoscepasdehongosdepudricionblanca AT moralesandrea elaboracionenlaboratoriodeunsistemasumergidodeproducciondeendo14bglucanasasecretadapordoscepasdehongosdepudricionblanca AT congostgabriela elaboracionenlaboratoriodeunsistemasumergidodeproducciondeendo14bglucanasasecretadapordoscepasdehongosdepudricionblanca AT fonsecamariai elaboracionenlaboratoriodeunsistemasumergidodeproducciondeendo14bglucanasasecretadapordoscepasdehongosdepudricionblanca AT abatecarlos elaboracionenlaboratoriodeunsistemasumergidodeproducciondeendo14bglucanasasecretadapordoscepasdehongosdepudricionblanca AT zapatapedrod elaboracionenlaboratoriodeunsistemasumergidodeproducciondeendo14bglucanasasecretadapordoscepasdehongosdepudricionblanca AT villalbalaural elaboracionenlaboratoriodeunsistemasumergidodeproducciondeendo14bglucanasasecretadapordoscepasdehongosdepudricionblanca |
Indice Volumen I Numero 1