CHARACTERIZATION OF AMINOPEPTIDASE N FROM STREPTOCOCCUS THERMOPHILUS TW43

Biochemical and kinetic characteristics of the general Aminopeptidase N (PepN) from Streptococcus thermophilus TW43 were studied. Maximal activity of the enzyme was determined at pH 6.8 and 40ºC. Pep N was strongly inhibited by EDTA and o-phenanthroline. Therefore, it was considered to be a metallop...

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Main Authors:	Marguet, Emilio R., Vallejo, Marisol, Pescuma, Micaela
Format:	Online
Language:	Spanish
Published:	Facultad de Ciencias Exactas, Químicas y Naturales 2006
Subjects:	Aminopeptidase N Streptococcus thermophilus Aminopeptidasa N
Online Access:	https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/448

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author	Marguet, Emilio R. Vallejo, Marisol Pescuma, Micaela
author_facet	Marguet, Emilio R. Vallejo, Marisol Pescuma, Micaela
author_sort	Marguet, Emilio R.
collection	Revista de Ciencia y Tecnologia RECyT
description	Biochemical and kinetic characteristics of the general Aminopeptidase N (PepN) from Streptococcus thermophilus TW43 were studied. Maximal activity of the enzyme was determined at pH 6.8 and 40ºC. Pep N was strongly inhibited by EDTA and o-phenanthroline. Therefore, it was considered to be a metallopeptidase. Imidazole partially inhibited enzyme activity suggesting histidine is one of the amino acids related to metal binding in the active site. Among the bivalent cations only Cu++ exhibited slight inhibition while Co++ increased enzymatic activity nearly fivefold. PepN remained active under ripening condition (pH 5.0 and 15ºC) suggesting that Streptococcus thermophilus TW43 may contribute to flavor cheese development through its role in production of free amino acids.
format	Online
id	ojs-article-448
institution	Facultad de Ciencias Exactas Quimicas y Naturales
language	Spanish
publishDate	2006
publisher	Facultad de Ciencias Exactas, Químicas y Naturales
record_format	ojs
spelling	ojs-article-4482020-03-06T00:43:30Z CHARACTERIZATION OF AMINOPEPTIDASE N FROM STREPTOCOCCUS THERMOPHILUS TW43 CARACTERIZACIÓN DE AMINOPEPTIDASA N DE STREPTOCOCCUS THERMOPHILUS TW43 Marguet, Emilio R. Vallejo, Marisol Pescuma, Micaela Aminopeptidase N Streptococcus thermophilus Aminopeptidasa N Streptococcus thermophilus Biochemical and kinetic characteristics of the general Aminopeptidase N (PepN) from Streptococcus thermophilus TW43 were studied. Maximal activity of the enzyme was determined at pH 6.8 and 40ºC. Pep N was strongly inhibited by EDTA and o-phenanthroline. Therefore, it was considered to be a metallopeptidase. Imidazole partially inhibited enzyme activity suggesting histidine is one of the amino acids related to metal binding in the active site. Among the bivalent cations only Cu++ exhibited slight inhibition while Co++ increased enzymatic activity nearly fivefold. PepN remained active under ripening condition (pH 5.0 and 15ºC) suggesting that Streptococcus thermophilus TW43 may contribute to flavor cheese development through its role in production of free amino acids. Se estudiaron las características bioquímicas y cinéticas de Aminopeptidasa N (PepN) de Streptococcus thermophilus TW43. La máxima actividad de la enzima fue determinada a pH 6,8 y 40ºC. El EDTA y la o-fenantrolina inhibieron fuertemente a PepN, en consecuencia se la consideró una metalopeptidasa. El imidazol inhibió parcialmente la actividad enzimática sugiriendo que la histidina es responsable de la unión del ión metálico en el sitio activo. Dentro de los cationes divalentes solo el Cu++ exhibió una leve inhibición, mientras que el Co++ aumentó la actividad enzimática aproximadamente cinco veces. La PepN se mantuvo activa bajo las condiciones de maduración sugiriendo que Streptococcus thermophilus TW43 puede contribuir al desarrollo del flavor en quesos a través de su función en la producción de aminoácidos libres. Facultad de Ciencias Exactas, Químicas y Naturales 2006-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/448 Argentine Journal of Science and Technology; Vol. 8 No. 1 (2006); 12-17 Revista de Ciencia y Tecnología; Vol. 8 Núm. 1 (2006); 12-17 1851-7587 0329-8922 spa https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/448/369 Derechos de autor 2006 Revista de Ciencia y Tecnología
spellingShingle	Aminopeptidase N Streptococcus thermophilus Aminopeptidasa N Streptococcus thermophilus Marguet, Emilio R. Vallejo, Marisol Pescuma, Micaela CHARACTERIZATION OF AMINOPEPTIDASE N FROM STREPTOCOCCUS THERMOPHILUS TW43
title	CHARACTERIZATION OF AMINOPEPTIDASE N FROM STREPTOCOCCUS THERMOPHILUS TW43
title_alt	CARACTERIZACIÓN DE AMINOPEPTIDASA N DE STREPTOCOCCUS THERMOPHILUS TW43
title_full	CHARACTERIZATION OF AMINOPEPTIDASE N FROM STREPTOCOCCUS THERMOPHILUS TW43
title_fullStr	CHARACTERIZATION OF AMINOPEPTIDASE N FROM STREPTOCOCCUS THERMOPHILUS TW43
title_full_unstemmed	CHARACTERIZATION OF AMINOPEPTIDASE N FROM STREPTOCOCCUS THERMOPHILUS TW43
title_short	CHARACTERIZATION OF AMINOPEPTIDASE N FROM STREPTOCOCCUS THERMOPHILUS TW43
title_sort	characterization of aminopeptidase n from streptococcus thermophilus tw43
topic	Aminopeptidase N Streptococcus thermophilus Aminopeptidasa N Streptococcus thermophilus
topic_facet	Aminopeptidase N Streptococcus thermophilus Aminopeptidasa N Streptococcus thermophilus
url	https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/448
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CHARACTERIZATION OF AMINOPEPTIDASE N FROM STREPTOCOCCUS THERMOPHILUS TW43

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