GENOMIC COMPARATIVE ANALYSIS BETWEEN PAPANICOLAU AND BLOOD SAMPLES FOR 5Q21 REGION MINISATELLITE
Papanicolaou is a bloodless technique that shows phenotypic changes related to tisular organization and differentiation. In this study we evaluated the relation between genomic instability of 5q21–5q22 minisatellite and the Papanicolaou. DNA of Papanicolaou and blood samples from 69 women were isola...
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| Format: | Online |
| Language: | Spanish |
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Facultad de Ciencias Exactas, Químicas y Naturales
2008
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| Subjects: | |
| Online Access: | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/456 |
| _version_ | 1810375039862702080 |
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| author | Cariaga Martínez, Ariel E. Rosiello, Selva Zapata, Pedro D. |
| author_facet | Cariaga Martínez, Ariel E. Rosiello, Selva Zapata, Pedro D. |
| author_sort | Cariaga Martínez, Ariel E. |
| collection | Revista de Ciencia y Tecnologia RECyT |
| description | Papanicolaou is a bloodless technique that shows phenotypic changes related to tisular organization and differentiation. In this study we evaluated the relation between genomic instability of 5q21–5q22 minisatellite and the Papanicolaou. DNA of Papanicolaou and blood samples from 69 women were isolated and amplified. No genetic changes in premalignant lesions were found. However, one allelic change was observed on DNA from cervix carcinoma. These findings may be related to a late change in the carcinogenesis process. The population analyzed was polymorphic showing 6 allelic forms (8–13) with heterozygosity rate of 0.33. |
| format | Online |
| id | ojs-article-456 |
| institution | Facultad de Ciencias Exactas Quimicas y Naturales |
| language | Spanish |
| publishDate | 2008 |
| publisher | Facultad de Ciencias Exactas, Químicas y Naturales |
| record_format | ojs |
| spelling | ojs-article-4562020-03-06T00:43:58Z GENOMIC COMPARATIVE ANALYSIS BETWEEN PAPANICOLAU AND BLOOD SAMPLES FOR 5Q21 REGION MINISATELLITE ANÁLISIS GENÓMICO COMPARATIVO ENTRE MUESTRAS DE PAPANICOLAU Y SANGRE PARA EL MINISATÉLITE UBICADO EN LA REGIÓN 5Q21 Cariaga Martínez, Ariel E. Rosiello, Selva Zapata, Pedro D. cervix carcinoma exfoliative cytology VNTR 5q21 minisatellite Cáncer de cuello de útero citología exfoliativa VNTR minisatélite 5q21 Papanicolaou is a bloodless technique that shows phenotypic changes related to tisular organization and differentiation. In this study we evaluated the relation between genomic instability of 5q21–5q22 minisatellite and the Papanicolaou. DNA of Papanicolaou and blood samples from 69 women were isolated and amplified. No genetic changes in premalignant lesions were found. However, one allelic change was observed on DNA from cervix carcinoma. These findings may be related to a late change in the carcinogenesis process. The population analyzed was polymorphic showing 6 allelic forms (8–13) with heterozygosity rate of 0.33. El Papanicolau es una de técnica incruenta que puede correlacionarse con el grado de organización y diferenciación tisular basándose en hallazgos fenotípicos. Este trabajo evalúa la relación entre la inestabilidad genómica de una secuencia minisatélite ubicada en la región 5q21–5q22 y las observaciones del Papanicolau en una población constituida por 69 mujeres. Para ello se amplificó la región minisatélite a partir de DNA proveniente de muestras para Papanicolau y se comparó con el proveniente de muestras sanguíneas. El análisis genómico comparativo reveló la presencia de una alteración compatible con la pérdida de una unidad de repetición en una muestra de carcinoma invasor de cuello uterino sin observarse cambios en muestras con lesiones premalignas. Este hallazgo podría indicar que las alteraciones genómicas en la región analizada son acontecimientos tardíos en la evolución tumoral. Se proponen los siguientes intervalos de referencia: sodio sérico (135–146 mMol/l), potasio sérico (3.4–5.0 mMol/l), sodio urinario (68–330 mMol/24 h) y potasio urinario (19–76 mMol/24 h). La población analizada fue polimórfica observándose 6 alelos (8–13) con una frecuencia de heterocigocidad de 0,33. Facultad de Ciencias Exactas, Químicas y Naturales 2008-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/456 Argentine Journal of Science and Technology; Vol. 10 No. 1 (2008); 12-17 Revista de Ciencia y Tecnología; Vol. 10 Núm. 1 (2008); 12-17 1851-7587 0329-8922 spa https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/456/377 Derechos de autor 2008 Revista de Ciencia y Tecnología |
| spellingShingle | cervix carcinoma exfoliative cytology VNTR 5q21 minisatellite Cáncer de cuello de útero citología exfoliativa VNTR minisatélite 5q21 Cariaga Martínez, Ariel E. Rosiello, Selva Zapata, Pedro D. GENOMIC COMPARATIVE ANALYSIS BETWEEN PAPANICOLAU AND BLOOD SAMPLES FOR 5Q21 REGION MINISATELLITE |
| title | GENOMIC COMPARATIVE ANALYSIS BETWEEN PAPANICOLAU AND BLOOD SAMPLES FOR 5Q21 REGION MINISATELLITE |
| title_alt | ANÁLISIS GENÓMICO COMPARATIVO ENTRE MUESTRAS DE PAPANICOLAU Y SANGRE PARA EL MINISATÉLITE UBICADO EN LA REGIÓN 5Q21 |
| title_full | GENOMIC COMPARATIVE ANALYSIS BETWEEN PAPANICOLAU AND BLOOD SAMPLES FOR 5Q21 REGION MINISATELLITE |
| title_fullStr | GENOMIC COMPARATIVE ANALYSIS BETWEEN PAPANICOLAU AND BLOOD SAMPLES FOR 5Q21 REGION MINISATELLITE |
| title_full_unstemmed | GENOMIC COMPARATIVE ANALYSIS BETWEEN PAPANICOLAU AND BLOOD SAMPLES FOR 5Q21 REGION MINISATELLITE |
| title_short | GENOMIC COMPARATIVE ANALYSIS BETWEEN PAPANICOLAU AND BLOOD SAMPLES FOR 5Q21 REGION MINISATELLITE |
| title_sort | genomic comparative analysis between papanicolau and blood samples for 5q21 region minisatellite |
| topic | cervix carcinoma exfoliative cytology VNTR 5q21 minisatellite Cáncer de cuello de útero citología exfoliativa VNTR minisatélite 5q21 |
| topic_facet | cervix carcinoma exfoliative cytology VNTR 5q21 minisatellite Cáncer de cuello de útero citología exfoliativa VNTR minisatélite 5q21 |
| url | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/456 |
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Structural Bioinformatics as a Complementary Tool for the Diagnosis and Treatment of Genetic-Based Diseases