| Summary: | Dermatophytes cause infections that do not adequately respond to antifungal treatment. In spite of this fact, worldwide susceptibility researches are scarce. The objective of this work was to evaluate the in vitro susceptibility of dermatophytes which were isolated from clinical samples facing three commonly used antifungals. Fifty dermatophytes strains of the following species were used: Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, Nannizzia gypsea, Trichophyton tonsurans and Epidermophyton floccosum. The antifungals tested were terbinafine, itraconazole, and fluconazole. Antifungal susceptibility testing was performed by using the broth microdilution technique and following the document M38-3rd ed. that belongs to the Clinical and Laboratory Standards Institute. The inoculum was adjusted at 103 CFU / ml. Plates were incubated at 35 ° C with ninety-six-hour daily readings. The terbinafine was the strongest antifungal drug (MIC values between 0.03 and 0.50 µg / ml), followed by the itraconazole (MIC values ranging from 0.12 to 4 µg / ml); while the fluconazole showed the weakest antifungal activity (MIC values from 8 to > 64 µg / ml). It can be concluded that the terbinafine has the best antifungal in vitro activity considering the dermatophyte strains under study.
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Antifungal capacity of aqueous extracts of ilex paraguariensis fruits against dermatophite fungi