Evaluation of the sensitivity of dermatophite fungi isolated from clinical samples to antifungals
Dermatophytes cause infections that do not adequately respond to antifungal treatment. In spite of this fact, worldwide susceptibility researches are scarce. The objective of this work was to evaluate the in vitro susceptibility of dermatophytes which were isolated from clinical samples facing three...
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| Format: | Online |
| Language: | Spanish |
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Facultad de Ciencias Exactas, Químicas y Naturales
2020
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| Subjects: | |
| Online Access: | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/586 |
| _version_ | 1824325857911504896 |
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| author | Mereles Rodriguez, Beda Elizabeth Fiedler, Jacqueline Bruquetas, Azucena Chade, Miriam |
| author_facet | Mereles Rodriguez, Beda Elizabeth Fiedler, Jacqueline Bruquetas, Azucena Chade, Miriam |
| author_sort | Mereles Rodriguez, Beda Elizabeth |
| collection | Revista de Ciencia y Tecnologia RECyT |
| description | Dermatophytes cause infections that do not adequately respond to antifungal treatment. In spite of this fact, worldwide susceptibility researches are scarce. The objective of this work was to evaluate the in vitro susceptibility of dermatophytes which were isolated from clinical samples facing three commonly used antifungals. Fifty dermatophytes strains of the following species were used: Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, Nannizzia gypsea, Trichophyton tonsurans and Epidermophyton floccosum. The antifungals tested were terbinafine, itraconazole, and fluconazole. Antifungal susceptibility testing was performed by using the broth microdilution technique and following the document M38-3rd ed. that belongs to the Clinical and Laboratory Standards Institute. The inoculum was adjusted at 103 CFU / ml. Plates were incubated at 35 ° C with ninety-six-hour daily readings. The terbinafine was the strongest antifungal drug (MIC values between 0.03 and 0.50 µg / ml), followed by the itraconazole (MIC values ranging from 0.12 to 4 µg / ml); while the fluconazole showed the weakest antifungal activity (MIC values from 8 to > 64 µg / ml). It can be concluded that the terbinafine has the best antifungal in vitro activity considering the dermatophyte strains under study. |
| format | Online |
| id | ojs-article-586 |
| institution | Facultad de Ciencias Exactas Quimicas y Naturales |
| language | Spanish |
| publishDate | 2020 |
| publisher | Facultad de Ciencias Exactas, Químicas y Naturales |
| record_format | ojs |
| spelling | ojs-article-5862021-03-10T12:37:39Z Evaluation of the sensitivity of dermatophite fungi isolated from clinical samples to antifungals Evaluación de la sensibilidad de hongos dermatofitos aislados de muestras clínicas a los antifúngicos Mereles Rodriguez, Beda Elizabeth Fiedler, Jacqueline Bruquetas, Azucena Chade, Miriam Ringworms dermatophytes antifungals susceptibility Tiñas dermatofitos antifúngicos sensibilidad Dermatophytes cause infections that do not adequately respond to antifungal treatment. In spite of this fact, worldwide susceptibility researches are scarce. The objective of this work was to evaluate the in vitro susceptibility of dermatophytes which were isolated from clinical samples facing three commonly used antifungals. Fifty dermatophytes strains of the following species were used: Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, Nannizzia gypsea, Trichophyton tonsurans and Epidermophyton floccosum. The antifungals tested were terbinafine, itraconazole, and fluconazole. Antifungal susceptibility testing was performed by using the broth microdilution technique and following the document M38-3rd ed. that belongs to the Clinical and Laboratory Standards Institute. The inoculum was adjusted at 103 CFU / ml. Plates were incubated at 35 ° C with ninety-six-hour daily readings. The terbinafine was the strongest antifungal drug (MIC values between 0.03 and 0.50 µg / ml), followed by the itraconazole (MIC values ranging from 0.12 to 4 µg / ml); while the fluconazole showed the weakest antifungal activity (MIC values from 8 to > 64 µg / ml). It can be concluded that the terbinafine has the best antifungal in vitro activity considering the dermatophyte strains under study. Los dermatofitos causan infecciones que en ocasiones no responden adecuadamente al tratamiento antifúngico implementado, sin embargo, se han publicado muy pocos estudios de sensibilidad a nivel mundial. El objetivo de este trabajo fue evaluar in vitro, la sensibilidad de hongos dermatofitos aislados de muestras clínicas frente a tres antifúngicos de uso habitual. Se trabajó con 50 cepas de las especies: Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, Nannizzia gypsea, Trichophyton tonsurans y Epidermophyton floccosum. Los antifúngicos probados fueron terbinafina, itraconazol y fluconazol. Para el estudio de la sensibilidad se desarrolló la técnica de microdilución en caldo según el documento M38-3ra ed. del Clinical and Laboratory Standards Institute. El inóculo fue ajustado a 103 UFC/ml. Se incubaron a 35 ºC con lecturas diarias hasta las 96 horas. Terbinafina fue la de mayor actividad antifungica (CIM entre 0,03 y 0,50 µg/ml), seguida por itraconazol (CIM entre 0,12 y 4 µg/ml). Fluconazol mostró la menor actividad antifúngica (CIM de 8 a > 64 µg/ml). Se concluye que la terbinafina presenta la mayor actividad antifúngica in vitro frente a las cepas de dermatofitos estudiadas. Facultad de Ciencias Exactas, Químicas y Naturales 2020-11-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/586 10.36995/j.recyt.2020.34.014 Argentine Journal of Science and Technology; Vol. 34 No. 1 (2020); 101-106 Revista de Ciencia y Tecnología; Vol. 34 Núm. 1 (2020); 101-106 1851-7587 0329-8922 spa https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/586/615 https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/586/630 Derechos de autor 2020 Beda Elizabeth Mereles Rodriguez, Jacqueline Fiedler, Azucena Bruquetas, Miriam Chade |
| spellingShingle | Ringworms dermatophytes antifungals susceptibility Tiñas dermatofitos antifúngicos sensibilidad Mereles Rodriguez, Beda Elizabeth Fiedler, Jacqueline Bruquetas, Azucena Chade, Miriam Evaluation of the sensitivity of dermatophite fungi isolated from clinical samples to antifungals |
| title | Evaluation of the sensitivity of dermatophite fungi isolated from clinical samples to antifungals |
| title_alt | Evaluación de la sensibilidad de hongos dermatofitos aislados de muestras clínicas a los antifúngicos |
| title_full | Evaluation of the sensitivity of dermatophite fungi isolated from clinical samples to antifungals |
| title_fullStr | Evaluation of the sensitivity of dermatophite fungi isolated from clinical samples to antifungals |
| title_full_unstemmed | Evaluation of the sensitivity of dermatophite fungi isolated from clinical samples to antifungals |
| title_short | Evaluation of the sensitivity of dermatophite fungi isolated from clinical samples to antifungals |
| title_sort | evaluation of the sensitivity of dermatophite fungi isolated from clinical samples to antifungals |
| topic | Ringworms dermatophytes antifungals susceptibility Tiñas dermatofitos antifúngicos sensibilidad |
| topic_facet | Ringworms dermatophytes antifungals susceptibility Tiñas dermatofitos antifúngicos sensibilidad |
| url | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/586 |
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Antifungal capacity of aqueous extracts of ilex paraguariensis fruits against dermatophite fungi