Selection and Characterization of α-L-Rhamnosidase Activity Producers of Psychrotolerants Strains
α-L-rhamnosidase plays a key role in grape juice and derived beverages processing by enhancing the aroma and in citric juices by removing bitterness. The aim of this work was to study and partially characterize marine microorganisms from different coastal areas of the Beagle Channel and intestinal c...
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| Format: | Online |
| Language: | Spanish |
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Facultad de Ciencias Exactas, Químicas y Naturales
2015
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| Subjects: | |
| Online Access: | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/360 |
| _version_ | 1813809292987334656 |
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| author | Alvarenga, Adriana E. Arévalo, María I. Castro, Guillermo R. Abate, Carlos M. |
| author_facet | Alvarenga, Adriana E. Arévalo, María I. Castro, Guillermo R. Abate, Carlos M. |
| author_sort | Alvarenga, Adriana E. |
| collection | Revista de Ciencia y Tecnologia RECyT |
| description | α-L-rhamnosidase plays a key role in grape juice and derived beverages processing by enhancing the aroma and in citric juices by removing bitterness. The aim of this work was to study and partially characterize marine microorganisms from different coastal areas of the Beagle Channel and intestinal contents of benthic organisms with α-L-rhamnosidase activity. A total of 10 microorganisms were selected because of their proper growth in MMB supplemented with rhamnose as the sole carbon source, at 8 and 20 °C. According to the quantitative determination of the α-L-rhamnosidase activity, G33 isolate showed the highest activity values at 37 °C (411.7 U.L-1), followed by F6 isolate at 30 °C (250.2 U.L-1). Molecular identification methods classified the isolates F6 and G33 as Serratia and Pseudomonas genera respectively. The results showed that α-L-rhamnosidase enzymes from Serratia sp. F6 and Pseudomonas sp. G33 strains are promising to be used in biotechnological strategies. |
| format | Online |
| id | ojs-article-360 |
| institution | Facultad de Ciencias Exactas Quimicas y Naturales |
| language | Spanish |
| publishDate | 2015 |
| publisher | Facultad de Ciencias Exactas, Químicas y Naturales |
| record_format | ojs |
| spelling | ojs-article-3602020-03-06T10:55:27Z Selection and Characterization of α-L-Rhamnosidase Activity Producers of Psychrotolerants Strains Selección y Caracterización de Cepas Psicrotolerantes Productoras de Actividad α-L-Ramnosidasa Alvarenga, Adriana E. Arévalo, María I. Castro, Guillermo R. Abate, Carlos M. α-L-rhamnosidase Pseudomonas sp. G33 Serratia sp. F6 Beagle Channel α-L-ramnosidas Pseudomonas sp. G33 Serratia sp. F6 Canal de Beagle α-L-rhamnosidase plays a key role in grape juice and derived beverages processing by enhancing the aroma and in citric juices by removing bitterness. The aim of this work was to study and partially characterize marine microorganisms from different coastal areas of the Beagle Channel and intestinal contents of benthic organisms with α-L-rhamnosidase activity. A total of 10 microorganisms were selected because of their proper growth in MMB supplemented with rhamnose as the sole carbon source, at 8 and 20 °C. According to the quantitative determination of the α-L-rhamnosidase activity, G33 isolate showed the highest activity values at 37 °C (411.7 U.L-1), followed by F6 isolate at 30 °C (250.2 U.L-1). Molecular identification methods classified the isolates F6 and G33 as Serratia and Pseudomonas genera respectively. The results showed that α-L-rhamnosidase enzymes from Serratia sp. F6 and Pseudomonas sp. G33 strains are promising to be used in biotechnological strategies. Las α-L-ramnosidasas desempeñan un rol clave en jugos de uvas y bebidas derivadas aumentando su aroma; y en jugos cítricos eliminando su amargor. El objetivo del trabajo fue el estudio y caracterización parcial de microorganismos marinos procedentes de diferentes zonas costeras del Canal de Beagle y de contenidos intestinales de organismos bentónicos con actividad α-L-ramnosidasa. A partir de 58 aislamientos cultivados en medio mineral de Brunner (MMB) con ramnosa, fueron seleccionados 10 microorganismos por presentar buen crecimiento a 8 y 20 ºC. A través de la determinación cuantitativa de la actividad α-L-ramnosidasa, se demostró que los aislamientos F6 y G33 presentaron los mayores valores de actividad a 30 °C (250,2 U.L-1) y a 37 °C (411,7 U.L-1). Los aislamientos seleccionados fueron identificados molecularmente como pertenecientes a los géneros Serratia y Pseudomonas. Los resultados de este trabajo indican que las enzimas α-L-ramnosidasas presentes en las cepas Serratia sp. F6 y Pseudomonas sp. G33 tienen un uso potencial en procesos biotecnológicos. Facultad de Ciencias Exactas, Químicas y Naturales 2015-12-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/360 Argentine Journal of Science and Technology; Vol. 24 No. 1 (2015); 45-54 Revista de Ciencia y Tecnología; Vol. 24 Núm. 1 (2015); 45-54 1851-7587 0329-8922 spa https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/360/264 Derechos de autor 2019 Revista de Ciencia y Tecnología |
| spellingShingle | α-L-rhamnosidase Pseudomonas sp. G33 Serratia sp. F6 Beagle Channel α-L-ramnosidas Pseudomonas sp. G33 Serratia sp. F6 Canal de Beagle Alvarenga, Adriana E. Arévalo, María I. Castro, Guillermo R. Abate, Carlos M. Selection and Characterization of α-L-Rhamnosidase Activity Producers of Psychrotolerants Strains |
| title | Selection and Characterization of α-L-Rhamnosidase Activity Producers of Psychrotolerants Strains |
| title_alt | Selección y Caracterización de Cepas Psicrotolerantes Productoras de Actividad α-L-Ramnosidasa |
| title_full | Selection and Characterization of α-L-Rhamnosidase Activity Producers of Psychrotolerants Strains |
| title_fullStr | Selection and Characterization of α-L-Rhamnosidase Activity Producers of Psychrotolerants Strains |
| title_full_unstemmed | Selection and Characterization of α-L-Rhamnosidase Activity Producers of Psychrotolerants Strains |
| title_short | Selection and Characterization of α-L-Rhamnosidase Activity Producers of Psychrotolerants Strains |
| title_sort | selection and characterization of α l rhamnosidase activity producers of psychrotolerants strains |
| topic | α-L-rhamnosidase Pseudomonas sp. G33 Serratia sp. F6 Beagle Channel α-L-ramnosidas Pseudomonas sp. G33 Serratia sp. F6 Canal de Beagle |
| topic_facet | α-L-rhamnosidase Pseudomonas sp. G33 Serratia sp. F6 Beagle Channel α-L-ramnosidas Pseudomonas sp. G33 Serratia sp. F6 Canal de Beagle |
| url | https://www.fceqyn.unam.edu.ar/recyt/index.php/recyt/article/view/360 |
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Table white wine manufactured with indigenous yeasts and Vitis labrusca from Cerro Azul Misiones